14.3.3 beta antibody (70R-13523)

Affinity purified Rabbit polyclonal 14.3.3 beta antibody

Synonyms Polyclonal 14.3.3 beta antibody, Anti-14.3.3 beta antibody, YWHAB antibody, protein 1054 antibody, brain protein 14.3.3 beta isoform antibody, protein kinase C inhibitor protein-1 antibody, 14-3-3 beta antibody, YWHAA antibody, KCIP-1 antibody, 14/3/3 beta antibody, 14.3.3 protein beta/alpha antibody, 14/3/3 beta, 14.3.3 beta, 14-3-3 beta, HS1 antibody, GW128 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB

Images

Western blot analysis using 14.3.3 beta antibody (70R-13523)

Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 12 % SDS PAGE gel and 14.3.3 beta antibody at a dilution of 1:10000

Specifications

Host Rabbit
Isotype IgG
Method of Purification 14.3.3 beta antibody was purified by antigen-affinity chromatography
Molecular Weight 28 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, IHC-P: 1:100-1:500, ICC:1:100-1:500, WB: 1:5000-1:20000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein belonging to the 14.3.3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using 14.3.3 beta antibody (70R-13523)</font></div>     | Western blot analysis of 20 ug of whole cell lysate (A: mouse brain) using a 12 % SDS PAGE gel and 14.3.3 beta antibody at a dilution of 1:10000
  • <div><font face=Calibri size=2>Western blot analysis using 14.3.3 beta antibody (70R-13523)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299; C: Hela; D: Hep G2; E: Molt-4; F: Raji) using a 12 % SDS PAGE gel and 14.3.3 beta antibody at a dilution of 1:10000
  • <div><font face=Calibri size=2>Immunofluorescent staining using 14.3.3 beta antibody (70R-13523)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using 14.3.3 beta antibody (70R-13523)</font></div>     | Immunohistochemical staining of paraffin-embedded SW480 xenograft using 14.3.3 beta antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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