14.3.3 sigma antibody (BSA/Azide Free) (10R-S101ap)
Mouse monoclonal 14.3.3 Sigma antibody (BSA/Azide Free)
Overview
Overview
| Synonyms | Monoclonal 14.3.3 Sigma antibody, Anti-14.3.3 Sigma antibody, 14/3/3 sigma, 14/3/3 sigma antibody, 14-3-3 sigma antibody, 14.3.3 sigma, 14-3-3 Sigma antibody, 14-3-3 sigma |
|---|---|
| Specificity | Human |
| Cross Reactivity | To be determined by end-user |
| Applications | IF, IHC-F, IHC-P, IP |
| Immunogen | 14.3.3 Sigma antibody (BSA/Azide-free) was raised in mouse using recombinant human 14-3-3 sigma protein as the immunogen. |
Images
Immunohistochemical staining using 14.3.3 sigma antibody (BSA/Azide Free) (10R-S101ap)
Formalin-fixed, paraffin-embedded human skin stained with 14.3.3 sigma antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis.
14.3.3 sigma antibody (BSA/Azide Free)
Immunohistochemical staining using 14.3.3 sigma antibody (BSA/Azide Free) (10R-S101ap)
Formalin-fixed, paraffin-embedded human skin stained with 14.3.3 sigma antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis.
Specifications
| Host | Mouse |
|---|---|
| Clone | 1433S01 |
| Isotype | IgG1 kappa |
| Method of Purification | 14.3.3 Sigma antibody (BSA/Azide Free) was purified by Protein G chromatography. |
| Molecular Weight | 26-30 kDa (predicted detection band MW) |
| Form & Buffer | Antibody purified from ascites fluid by protein G chromotography and supplied in 10mM PBS, pH 7.4. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | IHC-F: 2-4 ug/ml, IHC-P: 2-4 ug/ml, IP: 2 ug/ml protein lysate |
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Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. |
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