14-3-3 zeta antibody (70R-50563)

Purified Polyclonal 14-3-3 zeta antibody

Synonyms Polyclonal 14-3-3 zeta antibody, Anti-14-3-3 zeta antibody, 14 3 3 protein zeta/delta antibody, Protein kinase C inhibitor protein 1 antibody, KCIP-1 antibody
Specificity Recognizes endogenous levels of 14-3-3 zeta protein
Cross Reactivity Mouse, Rat, Bovine, Chicken, Pig
Applications ICC, IF, IHC, IP, WB
Immunogen 14-3-3 zeta antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 zeta as the immunogen

Images

Western blot analysis using 14-3-3 zeta antibody (70R-50563)

Western blot analysis of 14-3-3 zeta expression in HeLa (A); mouse brain (B); rat kidney (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification 14-3-3 zeta antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance 14 3 3 zeta is a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins.

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Images

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Western blot analysis using 14-3-3 zeta antibody (70R-50563)



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Western blot analysis of 14-3-3 zeta expression in HeLa (A); mouse brain (B); rat kidney (C) whole cell lysates.
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Immunohistochemical analysis using 14-3-3 zeta antibody (70R-50563)



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Immunohistochemical analysis of 14-3-3 zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using 14-3-3 zeta antibody (70R-50563)



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Immunofluorescent analysis of 14-3-3 zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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