14.3.3 zeta protein (His tag) (80R-1064)
Purified recombinant Human 14.3.3 zeta protein
|Synonyms||Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein protein, Tyr-3/Trp-5 monooxygenase Activation protein protein, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein protein, zeta polypeptide protein, Phospholipase A2 protein, Zeta, KCIP-1 protein, 14/3/3 zeta protein, 14-3-3 zeta, Protein kinase C inhibitor protein 1 protein, MGC111427 protein, 14-3-3 zeta protein, 14/3/3 zeta, 14.3.3 zeta, Tyr3/Trp5 monooxygenase Activation protein protein, 14-3-3 zeta protein, zeta polypeptide 14 3 3 protein zeta/delta protein, KCIP1 protein, YWHAZ protein, Zeta protein|
Coomassie Blue stained SDS-PAGE of 14.3.3 zeta protein (His tag) (80R-1064)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-245 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN|
|Grade & Purity||> 95% pure|
|Molecular Weight||32 kDa (245 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in PBS, pH 7.4.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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