160 kDa Neurofilament antibody (70R-10632)
Affinity purified Chicken polyclonal 160 kDa Neurofilament antibody
Overview
Overview
| Synonyms | Polyclonal 160 kDa Neurofilament antibody, Anti-160 kDa Neurofilament antibody, NF M antibody, NF-M antibody, Neurofilament Medium chain antibody, 160kD Neurofilament antibody, NFM antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | 160 kDa Neurofilament antibody was raised in Chicken using a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate corresponding to the NF-M gene product as the immunogen. |
Images
Immunofluorescent staining of 160 kDa Neurofilament antibody (70R-10632)
Dissociated cell culture prepared from an adult rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining. Picture courtesy of Dr. Gerry Shaw, University of Florida.
Specifications
| Host | Chicken |
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| Method of Purification | 160 kDa Neurofilament antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 2 mg/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:2000-1:5000; IHC: 1:2000-1:5000; WB: 1:5000-1:10000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Neurofilaments are the 10 nanometer (10nm) intermediate filaments found specifically in neurons. The subunits of neurofilaments are related structurally to the 10nm or intermediate filaments of other tissues such as the keratin subunits, which make 10nm filaments expressed specifically in epithelia. |
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