68 kDa Neurofilament antibody (70R-10631)
Affinity purified Chicken polyclonal 68 kDa Neurofilament antibody
Overview
Overview
| Synonyms | Polyclonal 68 kDa Neurofilament antibody, Anti-68 kDa Neurofilament antibody, NFL antibody, 68kD Neurofilament antibody, NF L antibody, Neurofilament Light chain antibody, NF-L antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | 68 kDa Neurofilament antibody was raised in Chicken using a synthetic peptide (Human P07196 and Mouse P08551) / keyhole limpet hemocyanin (KLH) conjugate corresponding to the NF-L gene product as the immunogen. |
Images
Immunohistochemical staining using 68 kDa Neurofilament antibody (70R-10631)
Tissue section through an adult mouse brain showing NFL (brown staining) of axons in the white matter of the cerebellum.
Specifications
| Host | Chicken |
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| Method of Purification | 68 kDa Neurofilament antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 300 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:1000-1:2000; IHC: 1:1000-1:2000; WB: 1:2000-1:5000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Neurofilaments are the 10 nanometer (10nm) intermediate filaments found specifically in neurons. The subunits of neurofilaments are related structurally to the 10nm or intermediate filaments of other tissues such as the keratin subunits, which make 10nm filaments expressed specifically in epithelia. |
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