A2LD1 protein (His tag) (80R-1717)
Purified recombinant Human A2LD1 protein
|Synonyms||A2LD 1, GGACT protein, A2LD1, A2LD 1 protein, A2LD-1 protein, A2LD-1, Gamma-glutamylaminecyclotransferase protein|
Coomassie Blue stained SDS-PAGE of A2LD1 protein (His tag) (80R-1717)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-153 amino acids: MGSSHHHHHH SSGLVPRGSH MALVFVYGTL KRGQPNHRVL RDGAHGSAAF RARGRTLEPY PLVIAGEHNI PWLLHLPGSG RLVEGEVYAV DERMLRFLDD FESCPALYQR TVLRVQLLED RAPGAEEPPA PTAVQCFVYS RATFPPEWAQ LPHHDSYDSE GPHGLRYNPR ENR|
|Grade & Purity||> 95% pure|
|Molecular Weight||19.4 kDa (173aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||A2LD1, also known as gamma-glutamylaminecyclotransferase, is an enzyme that converts gamma-glutamylamines to free amines and 5-oxoproline. It shows high activity toward gamma-glutamyl-epsilon-lysine, derived from the breakdown of fibrin and other proteins cross-linked by transglutaminases. This protein adopts the newly identified cyclotransferase fold, observed in gamma-glutamylcyclotransferase, an enzyme with activity toward gamma-glutamyl-alpha-amino acids. Recombinant human A2LD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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