AASDHPPT protein (His tag) (80R-1502)
Purified recombinant Human AASDHPPT protein
|Synonyms||LYS5 protein, CGI-80 protein, LYS2 protein, AASD-PPT protein, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase|
Coomassie Blue stained SDS-PAGE of AASDHPPT protein (His tag) (80R-1502)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||14-309 amino acids: MGSSHHHHHH SSGLVPRGSH MEGVRWAFSC GTWLPSRAEW LLAVRSIQPE EKERIGQFVF ARDAKAAMAG RLMIRKLVAE KLNIPWNHIR LQRTAKGKPV LAKDSSNPYP NFNFNISHQG DYAVLAAEPE LQVGIDIMKT SFPGRGSIPE FFHIMKRKFT NKEWETIRSF KDEWTQLDMF YRNWALKESF IKAIGVGLGF ELQRLEFDLS PLNLDIGQVY KETRLFLDGE EEKEWAFEES KIDEHHFVAV ALRKPDGSRH QDVPSQDDSK PTQRQFTILN FNDLMSSAVP MTPEDPSFWD CFCFTEEIPI RNGTKS|
|Grade & Purity||> 95% pure|
|Molecular Weight||36.4 kDa(316aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||AASDHPPT belongs to the P-Pant transferase superfamily. This protein catalyzes the post-translational modification of target proteins by phosphopantetheine and can transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN (in vitro). It is detected in heart, skeletal muscle, placenta, testis, brain, pancreas, liver and kidney. Recombinant human AASDHPPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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