AATF antibody (70R-50933)

Purified Polyclonal AATF antibody

Synonyms Polyclonal AATF antibody, Anti-AATF antibody, CHE1 antibody, DED antibody, Protein AATF antibody, Apoptosis-antagonizing transcription factor antibody, Rb-binding protein Che-1 antibody
Specificity Recognizes endogenous levels of AATF protein
Cross Reactivity Monkey
Applications ICC, IF, IHC, IP, WB
Immunogen AATF antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AATF as the immunogen

Images

Western blot analysis using AATF antibody (70R-50933)

Western blot analysis of AATF expression in HUVEC (A); HeLa (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification AATF antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance AATF may function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression.

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Images

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Western blot analysis using AATF antibody (70R-50933)



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Western blot analysis of AATF expression in HUVEC (A); HeLa (B) whole cell lysates.
  • 

Immunohistochemical analysis using AATF antibody (70R-50933)



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Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using AATF antibody (70R-50933)



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Immunofluorescent analysis of AATF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €323.11
Size: 100 ul
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