ABL1/2 antibody (70R-49323)

Purified Polyclonal ABL1/2 antibody

Synonyms Polyclonal ABL1/2 antibody, Anti-ABL1/2 antibody, ABL1 antibody, ABL antibody, JTK7 antibody, Tyrosine-protein kinase ABL1 antibody, Abelson murine leukemia viral oncogene homolog 1 antibody, Abelson tyrosine-protein kinase 1 antibody, Proto-oncogene c-Abl antibody, p150 antibody, ABL2 antibody, ABLL antibody, ARG antibody, Abelson tyrosine-protein kinase 2 antibody, Abelson murine leukemia viral oncogene homolog 2 antibody, Abelson-related gene protein antibody, Tyrosine-protein kinase ARG antibody
Specificity Recognizes endogenous levels of ABL1/2 protein
Cross Reactivity Mouse, Rat
Applications ICC, IF, IHC, WB
Immunogen ABL1/2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ABL1/2 as the immunogen

Images

Western blot analysis using ABL1/2 antibody (70R-49323)

Western blot analysis of ABL1/2 (pY393/439) expression in HeLa colchicine-treated (A); SP2/0 colchicine-treated (B); H9C2 colchicine-treated (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ABL1/2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ABL1/2 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics.

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Images

  • 

Western blot analysis using ABL1/2 antibody (70R-49323)



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Western blot analysis of ABL1/2 (pY393/439) expression in HeLa colchicine-treated (A); SP2/0 colchicine-treated (B); H9C2 colchicine-treated (C) whole cell lysates.
  • 

Immunohistochemical analysis using ABL1/2 antibody (70R-49323)



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Immunohistochemical analysis of ABL1/2 (pY393/439) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using ABL1/2 antibody (70R-49323)



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Immunofluorescent analysis of ABL1/2 (pY393/439) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €233.95
Size: 100 ul
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