ABTS Substrate (42-AB08S)
High sensitivity HRP microwell substrate (containing ABTS)
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user. This product should be allowed to reach room temperature (25C) prior to use. For immunoassay microwell applications, 100 ul of substrate solution is added to each well producing a soluble, blue-green product. For best results, sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units. The substrate reaction can be stopped using equal volumes of 1% of sodium dodecyl sulfate (SDS). Sample OD values can be read with or without the addition of stop solution in the 405-410 nm range. Dilution of the substrate is not recommended. To reduce the intensity of the reaction, it is recommended that the antibodies or conjugates be diluted. |
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Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | This HRP Microwell Substrate contains 2.2’-azino-di[3-ethylbenzthiazoline sulfonate] in a mild acidic buffer. The substrate is supplied as a one component ready to use solution. Unreacted substrate should be colorless to very light green in appearance. When this substrate system is reacted with peroxidase, a soluble blue-green reaction product is obtained. |
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