ACADM protein (His tag) (80R-1366)
Purified recombinant Human ACADM protein
|Synonyms||FLJ18227 protein, CAD protein, FLJ93013 protein, MCAD protein, FLJ99884 protein, Medium chain specific acyl CoA dehydrogenase protein, ACAD1 protein, Medium chain specific acyl CoA dehydrogenase mitochondrial, ACAD 1 protein, MCADH protein, Medium chain acyl CoA dehydrogenase protein, Medium chain fatty acyl CoA dehydrogenase protein, Acyl Coenzyme A Dehydrogenase protein, Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain protein, Medium-chain specific acyl-CoA dehydrogenase protein, C4 to C12 Straight Chain protein|
Coomassie Blue stained SDS-PAGE of ACADM protein (His tag) (80R-1366)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||26-421 amino acids: MGSSHHHHHH SSGLVPRGSH MKANRQREPG LGFSFEFTEQ QKEFQATARK FAREEIIPVA AEYDKTGEYP VPLIRRAWEL GLMNTHIPEN CGGLGLGTFD ACLISEELAY GCTGVQTAIE GNSLGQMPII IAGNDQQKKK YLGRMTEEPL MCAYCVTEPG AGSDVAGIKT KAEKKGDEYI INGQKMWITN GGKANWYFLL ARSDPDPKAP ANKAFTGFIV EADTPGIQIG RKELNMGQRC SDTRGIVFED VKVPKENVLI GDGAGFKVAM GAFDKTRPVV AAGAVGLAQR ALDEATKYAL ERKTFGKLLV EHQAISFMLA EMAMKVELAR MSYQRAAWEV DSGRRNTYYA SIAKAFAGDI ANQLATDAVQ ILGGNGFNTE YPVEKLMRDA KIYQIYEGTS QIQRLIVARE HIDKYKN|
|Grade & Purity||> 90% pure|
|Molecular Weight||45.9 kDa (417aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ACADM, also known as acyl-Coenzyme A dehydrogenase is an enzyme that is important for breaking down (degrading) a certain group of fats called medium-chain fatty acids. It is essential for converting these particular fatty acids to energy, especially during periods without food (fasting). This protein functions in mitochondria, the energy-producing centers within cells. It is found in the mitochondria of several types of tissues, particularly the liver. Recombinant human ACADM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques|
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