ACAT1 antibody (70R-49325)

Purified Polyclonal ACAT1 antibody

Synonyms Polyclonal ACAT1 antibody, Anti-ACAT1 antibody, ACAT antibody, MAT antibody, Acetyl-CoA acetyltransferase mitochondrial antibody, Acetoacetyl-CoA thiolase antibody, T2 antibody
Specificity Recognizes endogenous levels of ACAT1 protein
Cross Reactivity Mouse, Rat, Monkey
Applications IHC, IP, WB
Immunogen ACAT1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACAT1 as the immunogen

Images

Western blot analysis using ACAT1 antibody (70R-49325)

Western blot analysis of ACAT1 expression in HeLa (A); HEK293T (B); SP2/0 (C); H9C2 (D); THP1 (E) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ACAT1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA.

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Images

  • 

Western blot analysis using ACAT1 antibody (70R-49325)



 | 

Western blot analysis of ACAT1 expression in HeLa (A); HEK293T (B); SP2/0 (C); H9C2 (D); THP1 (E) whole cell lysates.
  • 

Immunohistochemical analysis using ACAT1 antibody (70R-49325)



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Immunohistochemical analysis of ACAT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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