AChE antibody (70R-12595)

Affinity purified Rabbit polyclonal AChE antibody

Synonyms Polyclonal AChE antibody, Anti-AChE antibody, N-ACHE antibody, acetylcholinesterase antibody, apoptosis-related acetylcholinesterase antibody, ARACHE antibody, YT antibody, ACHE antibody, Yt blood group antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen AChE antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 406 and 614 of AChE

Images

Immunofluorescent staining using AChE antibody (70R-12595)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.

Specifications

Host Rabbit
Isotype IgG
Method of Purification AChE antibody was purified by antigen-affinity chromatography
Molecular Weight 68 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using AChE antibody (70R-12595)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
  • <div><font face=Calibri size=2>Western blot analysis using AChE antibody (70R-12595)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 7.5 % SDS PAGE gel and AChE antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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