ackA protein (His tag) (80R-3979)
Recombinant E.coli ackA protein
Overview
Overview
| Synonyms | Recombinant ackA protein Acetate kinase A and propionate kinase 2 protein, ECK2290 protein, JW2293 protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie blue stained SDS-PAGE showing analysis of ackA protein (His tag) (80R-3979)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-400 aa; MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 45.7 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1 mM DTT |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for 1-2 weeks. For long term storage, aliquot and store at -20 to -70 deg C. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | ackA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E.coli ackA protein, fused to His-tag at N-terminus, was expressed in E.coli |
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