Actin antibody (10R-C116a)

Mouse monoclonal Actin antibody

Synonyms Monoclonal Actin antibody, Anti-Actin antibody, b-actin antibody, ACTB antibody, beta Actin antibody, beta-actin antibody
Cross Reactivity Bovine, chicken, human, rabbit
Applications ELISA, IHC-F, IHC-P, WB
Immunogen Actin antibody was raised in mouse using synthetic NH2 terminus decapeptide of cardiac isoform of actin as the immunogen.

Specifications

Host Mouse
Clone AC1-20.4.2
Isotype IgG1
Method of Purification Protein G affinity chromatography
Form & Buffer Lyophilized. Reconstitute in 1 ml dist. water (final solution contains 0.09 % NaN3, 0.5% BSA in PBS buffer, pH 7.4)

Usage & Assay Information

Usage Recommendations ELISA: 1:500, IHC-F: 1:10, IHC-P: 1:10, WB:1:1,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Actin is a globular, roughly 42- kDa protein found in all eukaryotic cells where it may be present at concentrations of over 100 uM. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells.

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Availability: In stock

Price: €277.55
Size: 50 ug
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