Actin antibody (biotin) (61R-A108aBT)
Mouse monoclonal Actin antibody (biotin)
Overview
Overview
| Synonyms | Monoclonal Actin antibody, Anti-Actin antibody, b-actin antibody, beta-actin antibody, ACTB antibody, beta Actin antibody |
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| Cross Reactivity | Baboon, chicken, cow, human, mouse, rabbit, rat |
| Applications | IF, IHC-F, IHC-P |
| Immunogen | Actin antibody (biotin) was raised in mouse using N-Terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at the N-terminus, as the immunogen. |
Images
Immunohistochemical staining using Actin antibody (biotin) (61R-A108aBT)
Formalin-fixed, paraffin-embedded human leiomyoma stained with Actin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of smooth muscle cells.
Specifications
| Host | Mouse |
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| Clone | 1A4 |
| Isotype | IgG2a kappa |
| Method of Purification | Actin antibody (biotin) was purified by Protein G chromatography. |
| Tag/Conjugate | Biotin |
| Form & Buffer | Antibody purified from ascites fluid by protein G chromotography and supplied in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% NaN3. |
| Concentration | 40 ug/ml |
Usage & Assay Information
| Usage Recommendations | IHC-F: 1:800, IHC-P: 1:800 |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling. |
General Information
| Biological Significance | Actin is a globular, roughly 42- kDa protein found in eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. |
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