ACTN1 protein (80R-4307)
Purified Recombinant ACTN1 protein (His tagged)
Overview
Overview
| Synonyms | BDPLT15 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis using ACTN1 protein (His tag) (80R-4307)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-249 aa: MGSSHHHHHH SSGLVPRGSH MGSEFMDHYD SQQTNDYMQP EEDWDRDLLL DPAWEKQQRK TFTAWCNSHL RKAGTQIENI EEDFRDGLKL MLLLEVISGE RLAKPERGKM RVHKISNVNK ALDFIASKGV KLVSIGAEEI VDGNVKMTLG MIWTIILRFA IQDISVEETS AKEGLLLWCQ RKTAPYKNVN IQNFHISWKD GLGFCALIHR HRPELIDYGK LRKDDPLTNL NTAFDVAEKY LDIPKMLDAE DIVGTARPDE KAIMTYVSSF YHAF |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 31.4 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in PBS buffer (pH 7.4) containing 10% glycerol |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | ACTN1 also known as alpha-actinin-1 isoform a. ACTN1 functions as an actin-binding-protein. ACTN1 determines the motility of keratinocytes by regulating the organization of the actin cytoskeleton, focal adhesion, and hemidesmosome proteins complexes, thereby modulating cell speed, lamellipodial dynamics, and directed migration. Recombinant human ACTN1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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