ACTN2 antibody (70R-49331)

Purified Polyclonal ACTN2 antibody

Synonyms Polyclonal ACTN2 antibody, Anti-ACTN2 antibody, ACTN2 antibody, Alpha-actinin-2 antibody, Alpha-actinin skeletal muscle isoform 2 antibody, F-actin cross-linking protein antibody, ACTN3 antibody, Alpha-actinin-3 antibody, Alpha-actinin skeletal muscle isoform 3 antibody, F-actin cross-linking protein antibody
Specificity Recognizes endogenous levels of ACTN2 protein
Cross Reactivity Mouse, Rat, Bovine, Cat
Applications ICC, IF, IHC, IP, WB
Immunogen ACTN2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN2 as the immunogen

Images

Western blot analysis using ACTN2 antibody (70R-49331)

Western blot analysis of ACTN2 expression in HeLa (A); mouse kidney (B); H9C2 (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ACTN2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ACTN2 is a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

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Images

  • 

Western blot analysis using ACTN2 antibody (70R-49331)



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Western blot analysis of ACTN2 expression in HeLa (A); mouse kidney (B); H9C2 (C) whole cell lysates.
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Immunohistochemical analysis using ACTN2 antibody (70R-49331)



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Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using ACTN2 antibody (70R-49331)



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Immunofluorescent analysis of ACTN2 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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