ACTN3 antibody (70R-49332)

Purified Polyclonal ACTN3 antibody

Synonyms Polyclonal ACTN3 antibody, Anti-ACTN3 antibody, Alpha-actinin-3 antibody, Alpha-actinin skeletal muscle isoform 3 antibody, F-actin cross-linking protein antibody
Specificity Recognizes endogenous levels of ACTN3 protein
Cross Reactivity Mouse, Rat, Bovine, Rabbit
Applications ICC, IF, WB
Immunogen ACTN3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN3 as the immunogen

Images

Western blot analysis using ACTN3 antibody (70R-49332)

Western blot analysis of ACTN3 expression in HeLa (A); mouse muscle (B); rat muscle (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ACTN3 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ACTN3 is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments.

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Images

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Western blot analysis using ACTN3 antibody (70R-49332)



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Western blot analysis of ACTN3 expression in HeLa (A); mouse muscle (B); rat muscle (C) whole cell lysates.
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Immunofluorescent analysis using ACTN3 antibody (70R-49332)



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Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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