ADAR2 antibody (70R-49336)

Purified Polyclonal ADAR2 antibody

Synonyms Polyclonal ADAR2 antibody, Anti-ADAR2 antibody, ADAR2 antibody, DRADA2 antibody, RED1 antibody, Double-stranded RNA-specific editase 1 antibody, RNA-editing deaminase 1 antibody, RNA-editing enzyme 1 antibody, dsRNA adenosine deaminase antibody
Specificity Recognizes endogenous levels of ADAR2 protein
Cross Reactivity Mouse, Rat, Chicken
Applications ICC, IF, IHC, WB
Immunogen ADAR2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADAR2 as the immunogen

Images

Western blot analysis using ADAR2 antibody (70R-49336)

Western blot analysis of ADAR2 expression in HepG2 (A); MCF7 (B); mouse brain (C); rat brain (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ADAR2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ADAR2 is a nuclear enzyme essential for GluR2 pre-mRNA editing.

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Images

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Western blot analysis using ADAR2 antibody (70R-49336)



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Western blot analysis of ADAR2 expression in HepG2 (A); MCF7 (B); mouse brain (C); rat brain (D) whole cell lysates.
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Immunofluorescent analysis using ADAR2 antibody (70R-49336)



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Immunofluorescent analysis of ADAR2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemical analysis using ADAR2 antibody (70R-49336)



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Immunohistochemical analysis of ADAR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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