Adenosine A2a Receptor antibody (70R-49342)

Purified Polyclonal Adenosine A2a Receptor antibody

Synonyms Polyclonal Adenosine A2a Receptor antibody, Anti-Adenosine A2a Receptor antibody, ADORA2 antibody, Adenosine receptor A2a antibody
Specificity Recognizes endogenous levels of Adenosine A2a Receptor protein
Cross Reactivity Mouse, Rat, Monkey
Applications ICC, IF, WB
Immunogen Adenosine A2a Receptor antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Adenosine A2a Receptor as the immunogen

Images

Western blot analysis using Adenosine A2a Receptor antibody (70R-49342)

Western blot analysis of Adenosine A2a Receptor expression in HeLa (A); Raw264.7 (B); H9C2 (C); rat kidney (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Adenosine A2a Receptor antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Adenosine A2a Receptor is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways.

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Images

  • 

Western blot analysis using Adenosine A2a Receptor antibody (70R-49342)



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Western blot analysis of Adenosine A2a Receptor expression in HeLa (A); Raw264.7 (B); H9C2 (C); rat kidney (D) whole cell lysates.
  • 

Immunofluorescent analysis using Adenosine A2a Receptor antibody (70R-49342)



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Immunofluorescent analysis of Adenosine A2a Receptor staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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