Adenylate Cyclase 5/6 antibody (70R-49338)

Purified Polyclonal Adenylate Cyclase 5/6 antibody

Synonyms Polyclonal Adenylate Cyclase 5/6 antibody, Anti-Adenylate Cyclase 5/6 antibody, ADCY5 antibody, Adenylate cyclase type 5 antibody, ATP pyrophosphate-lyase 5 antibody, Adenylate cyclase type V antibody, Adenylyl cyclase 5 antibody, ADCY6 antibody, KIAA0422 antibody, Adenylate cyclase type 6 antibody, ATP pyrophosphate-lyase 6 antibody, Adenylate cyclase type VI antibody, Adenylyl cyclase 6 antibody, Ca(2+)-inhibitable adenylyl cyclase antibody
Specificity Recognizes endogenous levels of Adenylate Cyclase 5/6 protein
Cross Reactivity Mouse, Rat, Dog, Monkey, Rabbit
Applications ICC, IF, WB
Immunogen Adenylate Cyclase 5/6 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenylate Cyclase 5/6 as the immunogen

Images

Western blot analysis using Adenylate Cyclase 5/6 antibody (70R-49338)

Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A); Raw264.7 (B); PC12 (C); rat brain (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Adenylate Cyclase 5/6 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Adenylate Cyclase 5/6 is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). The expression of this gene is found in normal thyroid and brain tissues, as well as some tumors; and its expression is significantly higher in one hyperfunctioning thyroid tumor than in normal thyroid tissue.

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Images

  • 

Western blot analysis using Adenylate Cyclase 5/6 antibody (70R-49338)



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Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A); Raw264.7 (B); PC12 (C); rat brain (D) whole cell lysates.
  • 

Immunofluorescent analysis using Adenylate Cyclase 5/6 antibody (70R-49338)



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Immunofluorescent analysis of Adenylate Cyclase 5/6 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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