Polyclonal Adenylate Cyclase 9 antibody, Anti-Adenylate Cyclase 9 antibody, KIAA0520 antibody, Adenylate cyclase type 9 antibody, ATP pyrophosphate-lyase 9 antibody, Adenylate cyclase type IX antibody, Adenylyl cyclase 9 antibody
Specificity
Recognizes endogenous levels of Adenylate Cyclase 9 protein
Cross Reactivity
Mouse, Rat
Applications
ICC, IF, IHC, WB
Immunogen
Adenylate Cyclase 9 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Adenylate Cyclase 9 as the immunogen
Western blot analysis using Adenylate Cyclase 9 antibody (70R-49339)
Western blot analysis of Adenylate Cyclase 9 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.
Adenylate Cyclase 9 antibody
Western blot analysis using Adenylate Cyclase 9 antibody (70R-49339)
Western blot analysis of Adenylate Cyclase 9 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.
Immunofluorescent analysis using Adenylate Cyclase 9 antibody (70R-49339)
Immunofluorescent analysis of Adenylate Cyclase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis using Adenylate Cyclase 9 antibody (70R-49339)
Immunohistochemical analysis of Adenylate Cyclase 9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Specifications
Host
Rabbit
Method of Purification
Adenylate Cyclase 9 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase, and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin, calcium, and somatostatin.