ADRB2 antibody (70R-51435)

Purified Polyclonal ADRB2 antibody

Synonyms Polyclonal ADRB2 antibody, Anti-ADRB2 antibody, ADRB2R antibody, B2AR antibody, Beta-2 adrenergic receptor antibody, Beta-2 adrenoreceptor antibody, Beta-2 adrenoceptor antibody
Specificity Recognizes endogenous levels of Beta-2 Adrenergic Receptor protein
Cross Reactivity Mouse, Rat, Bovine, Dog, Sheep
Applications FC, IHC, WB
Immunogen ADRB2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Beta-2 Adrenergic Receptor as the immunogen

Images

Western blot analysis using ADRB2 antibody (70R-51435)

Western blot analysis of Beta-2 Adrenergic Receptor expression in HepG2 (A); mouse kidney (B); rat kidney (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ADRB2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, FC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ADRB2 is directly associated with one of its ultimate effectors, the class C L-type calcium channel CaV1.2. This receptor-channel complex is coupled to the Gs G protein, which activates adenylyl cyclase, catalysing the formation of cyclic adenosine monophosphate (cAMP) which then activates protein kinase A, and the counterbalancing phosphatase PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling. A two-state biophysical and molecular model has been proposed to account for the pH and REDOX sensitivity of this and other GPCRs.

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Images

  • 

Western blot analysis using ADRB2 antibody (70R-51435)



 | 

Western blot analysis of Beta-2 Adrenergic Receptor expression in HepG2 (A); mouse kidney (B); rat kidney (C) whole cell lysates.
  • 

Immunohistochemical analysis using ADRB2 antibody (70R-51435)



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Immunohistochemical analysis of Beta-2 Adrenergic Receptor staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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