Aggrecan antibody (70R-49349)

Purified Polyclonal Aggrecan antibody

Synonyms Polyclonal Aggrecan antibody, Anti-Aggrecan antibody, AGC1 antibody, CSPG1 antibody, MSK16 antibody, Aggrecan core protein antibody, Cartilage-specific proteoglycan core protein antibody, CSPCP antibody, Chondroitin sulfate proteoglycan core protein 1 antibody, Chondroitin sulfate proteoglycan 1 antibody
Specificity Recognizes endogenous levels of Aggrecan protein
Cross Reactivity Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
Applications IHC, WB
Immunogen Aggrecan antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Aggrecan as the immunogen

Images

Western blot analysis using Aggrecan antibody (70R-49349)

Western blot analysis of Aggrecan expression in HeLa colchicine-treated (A); mouse kidney (B); PC12 colchicine-treated (C); NIH3T3 (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Aggrecan antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Aggrecan is a critical component for cartilage structure and the function of joints.

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Images

  • 

Western blot analysis using Aggrecan antibody (70R-49349)



 | 

Western blot analysis of Aggrecan expression in HeLa colchicine-treated (A); mouse kidney (B); PC12 colchicine-treated (C); NIH3T3 (D) whole cell lysates.
  • 

Immunohistochemical analysis using Aggrecan antibody (70R-49349)



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Immunohistochemical analysis of Aggrecan staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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