Immunofluorescent staining using AIMP1 antibody (70R-12881)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
AIMP1 antibody
Immunofluorescent staining using AIMP1 antibody (70R-12881)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Western blot analysis using AIMP1 antibody (70R-12881)
Western blot analysis of 30 ug of whole cell lysate (A:H1299; B:Hep G2) using a 12 % SDS PAGE gel and AIMP1 antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
AIMP1 antibody was purified by antigen-affinity chromatography
Molecular Weight
34 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells.