AK3 antibody (10R-1020)

Mouse monoclonal AK3 antibody

Synonyms Monoclonal AK3 antibody, Anti-AK3 antibody, adenylate kinase 3 like 1 antibody, Ak6 antibody, AK 3 antibody, adenylate kinase 6 antibody, AK -3, adenylate kinase antibody, AK -3 antibody, Ak3L1 antibody, AkL3L antibody, FIX antibody, AkL3L1 antibody, AK3, AK 3, adenylate kinase 3 antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IHC, WB
Immunogen AK3 antibody was raised in mouse using recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E. coli as the immunogen.

Images

Western Blot analysis of AK3 antibody (10R-1020)

The recombinant human Ak isozymes (Ak1, Ak2, Ak3) and mouse liver were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Ak3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone SJB3-36
Isotype IgG1 kappa
Source Anti-human Ak3 antibody, clone SJB3-36, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Ak3 protein.
Method of Purification AK3 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

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Images

  • Western Blot analysis of AK3 antibody (10R-1020) | The recombinant human Ak isozymes (Ak1, Ak2,   Ak3) and mouse liver were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Ak3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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