AKR1C1 antibody (70R-12909)

Affinity purified Rabbit polyclonal AKR1C1 antibody

Synonyms Polyclonal AKR1C1 antibody, Anti-AKR1C1 antibody, AKRC-1 antibody, MBAB antibody, HAKRC antibody, AKR1C1 antibody, H-37 antibody, dihydrodiol dehydrogenase 1/2 antibody, AKRC-1, AKRC 1, high-affinity hepatic bile acid-binding protein antibody, DDH antibody, 2-alpha-HSD antibody, AKR1C1, 20-alpha-hydroxysteroid dehydrogenase antibody, AKRC 1 antibody, 20-alpha-HSD antibody, C9 antibody, MGC8954 antibody, DDH1 antibody, trans-1 2-dihydrobenzene-1 2-diol dehydrogenase antibody, DD1 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen AKR1C1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 22 and 248 of AKR1C1

Images

Immunofluorescent staining using AKR1C1 antibody (70R-12909)

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification AKR1C1 antibody was purified by antigen-affinity chromatography
Molecular Weight 37 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using AKR1C1 antibody (70R-12909)</font></div>     | Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using AKR1C1 antibody (70R-12909)</font></div>     | Immunohistochemical staining of paraffin-embedded MDA-MB-468 xenograft using AKR1C1 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using AKR1C1 antibody (70R-12909)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 10 % SDS PAGE gel and AKR1C1 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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