Akt antibody (70R-37494)
Rabbit Polyclonal Akt antibody
Overview
Overview
| Synonyms | Polyclonal Akt antibody, Anti-Akt antibody, AKT1 antibody, AKT1 kinase antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, Protein kinase B antibody, Proto-oncogene c-Akt antibody, RAC antibody, rac protein kinase alpha antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, RAC-PK-alpha antibody, v-akt murine thymoma viral oncogene homolog 1 antibody |
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| Specificity | Akt antibody detects endogenous levels of total Akt protein |
| Cross Reactivity | Mouse, Rat |
| Applications | IHC, WB |
| Immunogen | Akt antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S) as the immunogen. |
Images
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | Akt antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:100-1:500, IHC: 1:50-1:100 |
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Storage & Safety
| Storage | Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major focus of attention because of its critical regulatory role in diverse cellular processes, including cancer progression and insulin metabolism. There are three highly related isoforms of Akt (Akt1, Akt2, and Akt3) and these represent the major signaling arm of PI3K. |
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