Akt antibody (70R-37494)

Rabbit Polyclonal Akt antibody

Synonyms Polyclonal Akt antibody, Anti-Akt antibody, AKT1 antibody, AKT1 kinase antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, Protein kinase B antibody, Proto-oncogene c-Akt antibody, RAC antibody, rac protein kinase alpha antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, RAC-PK-alpha antibody, v-akt murine thymoma viral oncogene homolog 1 antibody
Specificity Akt antibody detects endogenous levels of total Akt protein
Cross Reactivity Mouse, Rat
Applications IHC, WB
Immunogen Akt antibody was raised in Rabbit using a synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S) as the immunogen.

Images

Immunohistochemical staining using Akt antibody (70R-37494)

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Akt antibody

Specifications

Host Rabbit
Isotype IgG
Method of Purification Akt antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:100-1:500, IHC: 1:50-1:100

Storage & Safety

Storage Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major focus of attention because of its critical regulatory role in diverse cellular processes, including cancer progression and insulin metabolism. There are three highly related isoforms of Akt (Akt1, Akt2, and Akt3) and these represent the major signaling arm of PI3K.

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Images

  • Immunohistochemical staining using Akt antibody (70R-37494) | Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Akt antibody
  • Western blot analysis using Akt antibody (70R-37494) | Western blot analysis of extracts from HeLa cells using Akt antibody and Akt antibody (Lanes 3 and 4)

Availability: In stock

Price: €203.68
Size: 100 ug
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