AKT antibody (70R-49351)

Purified Polyclonal AKT antibody

Synonyms Polyclonal AKT antibody, Anti-AKT antibody, AKT1 antibody, PKB antibody, RAC antibody, RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, AKT2 antibody, RAC-beta serine/threonine-protein kinase antibody, Protein kinase Akt-2 antibody, Protein kinase B beta antibody, PKB beta antibody, RAC-PK-beta antibody, AKT3 antibody, PKBG antibody, RAC-gamma serine/threonine-protein kinase antibody, Protein kinase Akt-3 antibody, Protein kinase B gamma antibody, PKB gamma antibody, RAC-PK-gamma antibody, STK-2 antibody
Specificity Recognizes endogenous levels of AKT protein
Cross Reactivity Mouse, Rat, Bovine, Chicken
Applications ICC, IF, IP, WB
Immunogen AKT antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT as the immunogen

Images

Western blot analysis using AKT antibody (70R-49351)

Western blot analysis of AKT (pT308) expression in HeLa colchicine-treated (A); HL60 (B); NIH3T3 (C); SP2/0 colchicine-treated (D); PC12 colchicine-treated (E) whole cell lysates.

Specifications

Host Rabbit
Method of Purification AKT antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance AKT is involved in cellular survival pathways, by inhibiting apoptotic processes.

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Images

  • 

Western blot analysis using AKT antibody (70R-49351)



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Western blot analysis of AKT (pT308) expression in HeLa colchicine-treated (A); HL60 (B); NIH3T3 (C); SP2/0 colchicine-treated (D); PC12 colchicine-treated (E) whole cell lysates.
  • 

Immunofluorescent analysis using AKT antibody (70R-49351)



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Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €233.95
Size: 100 ul
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