AKT antibody (70R-49352)

Purified Polyclonal AKT antibody

Synonyms Polyclonal AKT antibody, Anti-AKT antibody, AKT1 antibody, PKB antibody, RAC antibody, RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, AKT2 antibody, RAC-beta serine/threonine-protein kinase antibody, Protein kinase Akt-2 antibody, Protein kinase B beta antibody, PKB beta antibody, RAC-PK-beta antibody, AKT3 antibody, PKBG antibody, RAC-gamma serine/threonine-protein kinase antibody, Protein kinase Akt-3 antibody, Protein kinase B gamma antibody, PKB gamma antibody, RAC-PK-gamma antibody, STK-2 antibody
Specificity Recognizes endogenous levels of AKT protein
Cross Reactivity Mouse, Rat, Bovine, Chicken
Applications ICC, IF, IHC, WB
Immunogen AKT antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT as the immunogen

Images

Western blot analysis using AKT antibody (70R-49352)

Western blot analysis of AKT (pY315) expression in HeLa (A); A549 (B); LOVO (C); HepG2 (D); NIH3T3 (E) whole cell lysates.

Specifications

Host Rabbit
Method of Purification AKT antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance AKT regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

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Images

  • 

Western blot analysis using AKT antibody (70R-49352)



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Western blot analysis of AKT (pY315) expression in HeLa (A); A549 (B); LOVO (C); HepG2 (D); NIH3T3 (E) whole cell lysates.
  • 

Immunohistochemical analysis using AKT antibody (70R-49352)



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Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using AKT antibody (70R-49352)



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Immunofluorescent analysis of AKT (pY315) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €233.95
Size: 100 ul
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