AKT antibody (70R-49355)

Purified Polyclonal AKT antibody

Synonyms Polyclonal AKT antibody, Anti-AKT antibody, AKT1 antibody, PKB antibody, RAC antibody, RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, AKT2 antibody, RAC-beta serine/threonine-protein kinase antibody, Protein kinase Akt-2 antibody, Protein kinase B beta antibody, PKB beta antibody, RAC-PK-beta antibody, AKT3 antibody, PKBG antibody, RAC-gamma serine/threonine-protein kinase antibody, Protein kinase Akt-3 antibody, Protein kinase B gamma antibody, PKB gamma antibody, RAC-PK-gamma antibody, STK-2 antibody
Specificity Recognizes endogenous levels of AKT protein
Cross Reactivity Mouse, Rat, Bovine, Chicken
Applications IHC, IP, WB
Immunogen AKT antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT as the immunogen

Images

Western blot analysis using AKT antibody (70R-49355)

Western blot analysis of AKT expression in A549 (A); NIH3T3 (B); mouse brain (C); rat brain (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification AKT antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance AKT is an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • 

Western blot analysis using AKT antibody (70R-49355)



 | 

Western blot analysis of AKT expression in A549 (A); NIH3T3 (B); mouse brain (C); rat brain (D) whole cell lysates.
  • 

Immunohistochemical analysis using AKT antibody (70R-49355)



 | 

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
OR
Shipping
View Our Distributors