AKT antibody (70R-49357)

Purified Polyclonal AKT antibody

Synonyms Polyclonal AKT antibody, Anti-AKT antibody, PKB antibody, RAC antibody, RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody
Specificity Recognizes endogenous levels of AKT protein
Cross Reactivity Mouse, Rat, Monkey
Applications IHC, WB
Immunogen AKT antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AKT as the immunogen

Images

Western blot analysis using AKT antibody (70R-49357)

Western blot analysis of AKT expression in HeLa (A); A549 (B); Raw264.7 (C); NIH3T3 (D); mouse lung (E); PC12 (F); rat heart (G) whole cell lysates.

Specifications

Host Rabbit
Method of Purification AKT antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance AKT is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The activation mechanism remains to be fully characterised but occurs downstream of phosphoinositide 3-kinase (PI-3K).

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Images

  • 

Western blot analysis using AKT antibody (70R-49357)



 | 

Western blot analysis of AKT expression in HeLa (A); A549 (B); Raw264.7 (C); NIH3T3 (D); mouse lung (E); PC12 (F); rat heart (G) whole cell lysates.
  • 

Immunohistochemical analysis using AKT antibody (70R-49357)



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Immunohistochemical analysis of AKT staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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