Western Blot analysis using Alcohol Dehydrogenase antibody (20-AR03)
Western blot analysis with Alcohol Dehydrogenase antibody used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM) 800 labeled Goat-anti-Rabbit IgG [H + L] diluted 1:5, 000 for 45 min at room temperature. The IRDye(TM) 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Alcohol Dehydrogenase antibody
Western Blot analysis using Alcohol Dehydrogenase antibody (20-AR03)
Western blot analysis with Alcohol Dehydrogenase antibody used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM) 800 labeled Goat-anti-Rabbit IgG [H + L] diluted 1:5, 000 for 45 min at room temperature. The IRDye(TM) 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specifications
Host
Rabbit
Method of Purification
Alcohol Dehydrogenase antibody was purified by delipidation and defibrination.
Form & Buffer
Lyophilized, Monospecific antisera, absorbed, delipidated and defibrinated, supplied in 0.02M NaPO4, 0.15M NaCl, pH 7.2, with 0.01% NaN4
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
General Information
Biological Significance
Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.