ALDH2 protein (30R-1265)

Purified recombinant Human ALDH2 protein

Synonyms Aldehyde dehydrogenase mitochondrial protein, ALDH E2 protein, mitochondrial Acetaldehyde dehydrogenase 2 protein, ALDH-2, Aldehyde dehydrogenase protein, ALDH 2 protein, ALDH 2 protein, ALDH-2 protein, ALDM protein, MGC1806 protein, ALDH 2, Aldehyde dehydrogenase 2 family protein, Mitochondrial aldehyde dehydrogenase 2 protein, ALDH class 2 protein, ALDH2, ALDHI protein, Nucleus encoded mitochondrial aldehyde dehydrogenase 2., Aldehyde dehydrogenase 2 family (mitochondrial) protein, ALDH-E2 protein, Liver mitochondrial ALDH protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA. 2. Equilibrate to 25 DEG C and monitor at A340nm until value is constant using a spectrophotometer. 3. Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes

Images

Coomassie Blue stained SDS-PAGE of ALDH2 protein (30R-1265)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 18-517 amino acids: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 54.5 kDa (501aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, 1mM EDTA, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is < 0.2 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 uM of acetaldehyde to acetic acid per minute at pH 8.0 at 25 DEG C in the pre

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of ALDH2 protein (30R-1265) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €235.21
Size: 100 ug
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