ALDOA antibody (70R-49363)

Purified Polyclonal ALDOA antibody

Synonyms Polyclonal ALDOA antibody, Anti-ALDOA antibody, ALDA antibody, Fructose-bisphosphate aldolase A antibody, Lung cancer antigen NY-LU-1 antibody, Muscle-type aldolase antibody
Specificity Recognizes endogenous levels of ALDOA protein
Cross Reactivity Mouse, Rat, Dog, Rabbit
Applications ICC, IF, IP, WB
Immunogen ALDOA antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ALDOA as the immunogen

Images

Western blot analysis using ALDOA antibody (70R-49363)

Western blot analysis of ALDOA expression in HEK293T (A); HepG2 (B); HeLa (C); NIH3T3 (D); PC12 (E); rat skeletal muscle (F) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ALDOA antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, IP: 1:10 - 1:100

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ALDOA plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • 

Western blot analysis using ALDOA antibody (70R-49363)



 | 

Western blot analysis of ALDOA expression in HEK293T (A); HepG2 (B); HeLa (C); NIH3T3 (D); PC12 (E); rat skeletal muscle (F) whole cell lysates.
  • 

Immunofluorescent analysis using ALDOA antibody (70R-49363)



 | 

Immunofluorescent analysis of ALDOA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
OR
Shipping
View Our Distributors