ALK1 antibody (70R-49334)

Purified Polyclonal ALK1 antibody

Synonyms Polyclonal ALK1 antibody, Anti-ALK1 antibody, ACVRLK1 antibody, ALK1 antibody, Serine/threonine-protein kinase receptor R3 antibody, SKR3 antibody, Activin receptor-like kinase 1 antibody, ALK-1 antibody, TGF-B superfamily receptor type I antibody, TSR-I antibody
Specificity Recognizes endogenous levels of ALK1 protein
Cross Reactivity Mouse, Rat, Bovine, Dog, Pig
Applications ICC, IF, IHC, WB
Immunogen ALK1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ALK1 as the immunogen

Images

Western blot analysis using ALK1 antibody (70R-49334)

Western blot analysis of ALK1 expression in MCF7 (A); mouse liver (B); rat liver (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ALK1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ALK1 is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail.

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Images

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Western blot analysis using ALK1 antibody (70R-49334)



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Western blot analysis of ALK1 expression in MCF7 (A); mouse liver (B); rat liver (C) whole cell lysates.
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Immunofluorescent analysis using ALK1 antibody (70R-49334)



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Immunofluorescent analysis of ALK1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemical analysis using ALK1 antibody (70R-49334)



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Immunohistochemical analysis of ALK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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