alpha 1 Antitrypsin antibody (biotin) (60R-AG003bt)
Goat polyclonal alpha 1 Antitrypsin antibody (biotin) conjugated
Overview
Overview
| Synonyms | Polyclonal alpha 1 antitrypsin antibody, Anti-alpha 1 antitrypsin antibody, alpha 1 protease inhibitor antibody, A1AT antibody, AAT antibody, alpha 1 antiproteinase antibody, Alpha-1 Antitrypsin antibody, Alpha 1 Anti Trypsin antibody |
|---|---|
| Specificity | Human alpha 1 Antitrypsin |
| Applications | ELISA, WB |
| Immunogen | alpha 1 Antitrypsin antibody (biotin) was raised in goat using human alpha-1-anti-Trypsin as the immunogen. |
Images
Western blot analysis using alpha 1 Antitrypsin antibody (biotin) (60R-AG003bt)
Western Blot of alpha 1 Antitrypsin antibody. y. Lane 1: alpha 1 Antitrypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. alpha 1 Antitrypsin antibody at 1:3000 for overnight at 4 deg C. Dylight 488 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking buffer overnight at 4 deg C. Size: 55kDa for alpha 1 Antitrypsin
Specifications
| Host | Goat |
|---|---|
| Method of Purification | alpha 1 Antitrypsin antibody (biotin) was purified by ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | Biotin |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:50,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Alpha 1-Antitrypsin or alpha1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily. It is generally known as serum trypsin inhibitor. Alpha 1-antitrypsin is also referred to as alpha-1 proteinase inhibitor (A1PI) because it inhibits a wide variety of proteases. |
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