alpha 1 Trypsin antibody (20R-AG002)

Goat polyclonal alpha 1 Trypsin antibody

Synonyms Polyclonal alpha 1 Trypsin antibody, Anti-alpha 1 Trypsin antibody, alpha Trypsin-1 antibody, A1AT antibody, alpha Trypsin 1 antibody, alpha 1 antiproteinase antibody, alpha Trypsin-1, alpha 1 Trypsin, AAT antibody, alpha Trypsin 1, alpha 1 antitrypsin antibody, alpha 1 protease inhibitor antibod
Specificity human alpha-1-trypsin
Cross Reactivity To be determined by end-user
Applications ELISA, WB
Immunogen Alpha-1 trypsin antibody was raised in goat using human alpha-1-anti-trypsin as the immunogen.

Images

Western blot analysis using alpha 1 Trypsin antibody (20R-AG002)

Western Blot of Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Each primary antibody was diluted to 1:1000 for overnight at 4 deg C. Rabbit IgG (H&L) antibody DyLight649 Conjugated (green), Goat IgG (H&L) antibody DyLight549 Conjugated (red), Mouse IgG (H&L) antibody DyLight488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4 deg C

Specifications

Host Goat
Method of Purification Alpha 1 Trypsin antibody was purified by ion exchange chromatography followed by dialysis.
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl and 0.01% NaN3.

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Alpha 1-Antitrypsin or alpha1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily. It is generally known as serum trypsin inhibitor. Alpha 1-antitrypsin is also referred to as alpha-1 proteinase inhibitor (A1PI) because it inhibits a wide variety of proteases.

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Images

  • Western blot analysis using alpha 1 Trypsin antibody (20R-AG002) | Western Blot of Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Each primary antibody was diluted to 1:1000 for overnight at 4 deg C. Rabbit IgG (H&L) antibody DyLight649 Conjugated (green), Goat IgG (H&L) antibody DyLight549 Conjugated (red), Mouse IgG (H&L) antibody DyLight488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4 deg C
  • Western blot analysis using alpha 1 Trypsin antibody (20R-AG002) | Western Blot of Trypsin antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: alpha 1 Antitrypsin (red), Transferrin, and Mouse anti GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Each primary antibody at 1:1000 for overnight at 4 deg C. DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: Blocking buffer at 30 min RT
  • Western blot analysis using alpha 1 Trypsin antibody (20R-AG002) | Western Blot of alpha 1 Antitrypsin antibody. Lane 1: alpha 1 Antitrypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. alpha 1 Antitrypsin antibody at 1:3000 for overnight at 4 deg C. Dylight 488 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking buffer overnight at 4 deg C. Size: 55kDa for a-1 Anti-Trypsin.

Availability: In stock

Price: €465.72
Size: 100 ug
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