Recognizes endogenous levels of Alpha-actin-3 protein
Cross Reactivity
Mouse, Rat, Bovine, Chicken, Rabbit
Applications
ICC, IF, IHC, WB
Immunogen
Alpha actin 3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin-3 as the immunogen
Western blot analysis using Alpha actin 3 antibody (70R-49330)
Western blot analysis of Alpha-actin-3 expression in DLD (A); HeLa (B); mouse brain (C); rat brain (D) whole cell lysates.
Alpha actin 3 antibody
Western blot analysis using Alpha actin 3 antibody (70R-49330)
Western blot analysis of Alpha-actin-3 expression in DLD (A); HeLa (B); mouse brain (C); rat brain (D) whole cell lysates.
Immunofluorescent analysis using Alpha actin 3 antibody (70R-49330)
Immunofluorescent analysis of Alpha-actin-3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis using Alpha actin 3 antibody (70R-49330)
Immunohistochemical analysis of Alpha-actin-3 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Specifications
Host
Rabbit
Method of Purification
Alpha actin 3 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide