alpha Tubulin 1A antibody (70R-13631)

Affinity purified Rabbit polyclonal alpha Tubulin 1A antibody

Synonyms Polyclonal alpha Tubulin 1A antibody, Anti-alpha Tubulin 1A antibody, hum-a-tub1 antibody, FLJ25113 antibody, tubulin alpha brain-specific antibody, tubulin alpha-1A chain antibody, alpha Tubulin A-1, LIS3 antibody, TUBA3 antibody, B-alpha-1 antibody, alpha Tubulin A 1 antibody, TUBA1A antibody, alpha Tubulin 1A, alpha Tubulin A 1, alpha Tubulin A-1 antibody, tubulin B-alpha-1 antibody, hum-a-tub2 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB

Images

Western blot analysis using alpha Tubulin 1A antibody (70R-13631)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and alpha Tubulin 1A antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification alpha Tubulin 1A antibody was purified by antigen-affinity chromatography
Molecular Weight 50 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.24 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using alpha Tubulin 1A antibody (70R-13631)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and alpha Tubulin 1A antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using alpha Tubulin 1A antibody (70R-13631)</font></div>     | Immunohistochemical staining of paraffin-embedded Breast ca using alpha Tubulin 1A antibody at a dilution of 1:250
  • <div><font face=Calibri size=2>Western blot analysis using alpha Tubulin 1A antibody (70R-13631)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: 293T; B: A431; C: JurKat; D: Raji) using a 10 % SDS PAGE gel and alpha Tubulin 1A antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using alpha Tubulin 1A antibody (70R-13631)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Tubulin 1A antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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