alpha Tubulin antibody (70R-13229)

Affinity purified Rabbit polyclonal alpha Tubulin antibody

Synonyms Polyclonal alpha Tubulin antibody, Anti-alpha Tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-1B chain antibody, alpha-tubulin ubiquitous antibody, tubulin alpha ubiquitous antibody, tubulin alpha-ubiquitous chain antibody, TUBA1B antibody, alpha tubulin antibody, K-alpha-1 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB

Images

Western blot analysis using alpha Tubulin antibody (70R-13229)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and alpha Tubulin antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification alpha Tubulin antibody was purified by antigen-affinity chromatography
Molecular Weight 50 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.81 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using alpha Tubulin antibody (70R-13229)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and alpha Tubulin antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using alpha Tubulin antibody (70R-13229)</font></div>     | Immunohistochemical staining of paraffin-embedded SW480 xenograft using alpha Tubulin antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using alpha Tubulin antibody (70R-13229)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10 % SDS PAGE gel and alpha Tubulin antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using alpha Tubulin antibody (70R-13229)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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