IHC: 1:100, WB: 1:500. For IHC, an incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Storage & Safety
Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
General Information
Biological Significance
alpha-methylacyl-CoA racemase (AMACR), (also known as P504S) is an enzyme that is involved in peroxisomal beta-oxidation of dietary branched-chain fatty acids. Recent studies have shown that, compared with expression in normal or benign prostate epithelium, AMACR is consistently overexpressed in prostate cancer epithelium, making it a specific marker for cancer cells within the prostate gland.