Amphiphysin protein (80-1531)

Purified Recombinant Amphiphysin protein (His tag) (GST tag)

Synonyms AMPH protein, AMPH1 protein, Amphiphysin protein
Species Human
Protein Type Recombinant
Applications ELISA, SDS-PAGE, WB

Images

Western Blot analysis using Amphiphysin protein (80-1418)

Specifications

Residues 1-695 aa
Expression System E.coli
Grade & Purity > 90% pure
Method of Purification Amphiphysin protein was purified using proprietary chromatographic techniques
Molecular Weight 109 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in 50 mM Tris buffer, pH 8.0, 150 mM NaCL, 10% glycerol, 1 mM EDTA and 2 mM DTT

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -70 deg c for long term storage. Avoid repeat freeze/thaw cycles

General Information

Product Use Purified Recombinant Amphiphysin protein for use in SDS-PAGE and Western Blot.
Biological Significance Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. Amphiphysin is a brain-enriched protein with an N-terminal lipid interaction, dimerisation and membrane bending BAR domain, a middle clathrin and adaptor binding domain and a C-terminal SH3 domain. In the brain, its primary function is thought to be the recruitment of dynamin to sites of clathrin-mediated endocytosis.
Expression Amphiphysin is expressed in neurons, certain endocrine cell types and spermatocytes. Amphiphysin is expressed in the cytoplasm; synaptic vesicle; synaptic vesicle membrane; cell junction and actin cytoskeleton of mammalian cells.
Implications in Disease Antibodies against AMPH are detected in patients with Stiff Person Syndrome (SPS), a rare disease of the central nervous system characterized by progressive rigidity of the body musculature with superimposed painful spasms. A subset of patients with SPS who were also affected by breast cancer are positive for autoantibodies against this protein.
Sequence Information Amphiphysin Is composed of 695 amino acids with a molecular weight of 109 kDa. There is a Phosphoserine residue at positions 506.
Database Links Entrez Gene: 273 Human, Omim: 600418 Human, SwissProt: P49418 Human, Unigene: 592182 Human

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Images

  • Western Blot analysis using Amphiphysin protein (80-1418) |

Availability: In stock

Price: €364.01
Size: 1 mg
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