Amphiphysin protein (80-1531)
Purified Recombinant Amphiphysin protein (His tag) (GST tag)
Overview
Overview
| Synonyms | AMPH protein, AMPH1 protein, Amphiphysin protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | ELISA, SDS-PAGE, WB |
Specifications
| Residues | 1-695 aa |
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| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Method of Purification | Amphiphysin protein was purified using proprietary chromatographic techniques |
| Molecular Weight | 109 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in 50 mM Tris buffer, pH 8.0, 150 mM NaCL, 10% glycerol, 1 mM EDTA and 2 mM DTT |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -70 deg c for long term storage. Avoid repeat freeze/thaw cycles |
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General Information
| Product Use | Purified Recombinant Amphiphysin protein for use in SDS-PAGE and Western Blot. |
|---|---|
| Biological Significance | Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. Amphiphysin is a brain-enriched protein with an N-terminal lipid interaction, dimerisation and membrane bending BAR domain, a middle clathrin and adaptor binding domain and a C-terminal SH3 domain. In the brain, its primary function is thought to be the recruitment of dynamin to sites of clathrin-mediated endocytosis. |
| Expression | Amphiphysin is expressed in neurons, certain endocrine cell types and spermatocytes. Amphiphysin is expressed in the cytoplasm; synaptic vesicle; synaptic vesicle membrane; cell junction and actin cytoskeleton of mammalian cells. |
| Implications in Disease | Antibodies against AMPH are detected in patients with Stiff Person Syndrome (SPS), a rare disease of the central nervous system characterized by progressive rigidity of the body musculature with superimposed painful spasms. A subset of patients with SPS who were also affected by breast cancer are positive for autoantibodies against this protein. |
| Sequence Information | Amphiphysin Is composed of 695 amino acids with a molecular weight of 109 kDa. There is a Phosphoserine residue at positions 506. |
| Database Links | Entrez Gene: 273 Human, Omim: 600418 Human, SwissProt: P49418 Human, Unigene: 592182 Human |
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