AMPK alpha 2 antibody (70R-12726)

Affinity purified Rabbit polyclonal AMPK alpha 2 antibody

Synonyms Polyclonal AMPK alpha 2 antibody, Anti-AMPK alpha 2 antibody, AMPK2 antibody, AMPK alpha 2 antibody, PRKAA2 antibody, AMPK alpha 2, AMPK-alpha-2 chain antibody, PRKAA antibody, AMPK alpha -2 antibody, AMPK antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody, 5'-AMP-activated protein kinase catalytic alpha-2 chain antibody, AMPK alpha 2, AMPK alpha -2
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen AMPK alpha 2 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2

Images

Western blot analysis using AMPK alpha 2 antibody (70R-12726)

Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 7.5 % SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification AMPK alpha 2 antibody was purified by antigen-affinity chromatography
Molecular Weight 62 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.55 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using AMPK alpha 2 antibody (70R-12726)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 7.5 % SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using AMPK alpha 2 antibody (70R-12726)</font></div>     | Immunohistochemical staining of paraffin-embedded A549 xenograft using AMPK alpha 2 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using AMPK alpha 2 antibody (70R-12726)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5 % SDS PAGE gel and AMPK alpha 2 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using AMPK alpha 2 antibody (70R-12726)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2 antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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