Monoclonal antibodies

Monoclonal antibodies for a target antigen on the other hand discriminate between specific epitopes thereby providing increased protection against specific disease organisms and cells.

Monoclonal antibodies are cells derived from a single clone and can be produced indefinitely. Theytarget various proteins that influence cell activity such as receptors or other proteins present on the surface of cancer and other diseased cells.

Monoclonals are produced when tumor cells with the ability to endlessly replicate are fused with mammalian cells that produce a specific antibody of interest which result in a fusion cell known as a hybridoma which will then continuously produce monoclonal antibodies, either in vitro or in vivo in a suitable host such as a mouse.

Fitzgerald Industries offers a huge range of over ten thousand monoclonal antibodies covering all areas of biochemical research and diagnostic medicine. We offer monoclonal antibodies for diseases such as cancer, cardiac markers, cell biology, metabolism, proteases, neuroscience, drugs of abuse and many more. Our monoclonals are of the highest quality and purity and are available in purified format or conjugated to a variety of useful fluorimetric, colorimetric and enzymatic tags such as biotin, horseradish peroxidase (HRP), alkaline phosphatase (AP), fluorescein isothiocyanate (FITC) and more.

Some of our most popular monoclonal antibodies include:

Insulin+Proinsulin antibody Suitable for ELISA and Immunohistochemistry, this protein A purified monoclonal is one of our most popular hormone antibodies.

This popularGAPDH antibody is used mainly as a loading control in Western blotting experiments. Biologically, GAPDH is a crucial metabolic enzyme, GAPDH catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules, and often researchers require very specific antibodies for cellular studies such as this GAPDH antibody which can be used in immunofluorence and IHC sections of cells and tissues as shown below.

Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH antibody (green), showing cytoplasmic localization.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining.

 

Streptococcus pneumoniae antibody  One of the most popular antibodies in our huge range of infectious disease monoclonals, this antibody to the bacterial meningitis causing S. Pneumonia is suitable for Immunofluorescence and ELISA procedures.

IFN gamma antibody (biotin)  IFN gamma is an anti-viral and anti-parasitic cytokine. This IFN gamma monoclonal is from our vast selection of conjugated monoclonals and is ideal for use in ELISA.

Vimentin antibody (FITC)  Another of our most popular conjugated monoclonals, this vimentin antibody is affinity purified, its FITC tag makes it an ideal choice for Immunofluorescence but it is also suitable for other immunoassays.

Check out our full range of monoclonal antibodies or simply search our website for your monoclonal of choice!