Immunofluorescence (IF) or (IFA) is a common laboratory technique used in almost all aspects of biotechnology. It is a form of light microscopy used mainly on microbiological samples utilizing the specificity of an antibody for its target protein or epitope to bind a molecule of fluorescent dye to a specific target targets within a cell or solution, following successful Immunofluorescence of a sample, it becomes possible to visualise the target molecule using a fluorescence microscope or automated reader.
Immunofluorescence is a highly flexible technique and is used on tissue sections, cultured cell lines, individual cells, and may be used to analyse the distribution of proteins, glycans, and small biological and non-biological molecules.
Like other forms of immunostaining, the two main methods used in Immunofluorescence are direct and indirect. Less frequently used is direct immunofluorescence whereby the antibody against the molecule of interest is chemically conjugated to a fluorescent dye. In indirect immunofluorescence, the primary antibody specific for the molecule of is unlabeled, and a second anti-immunoglobulin antibody directed the secondary antibody is tagged with the fluorescent dye, indirect Immunofluorescence is more sensitive.
Fitzgerald Industries supplies a vast range of primary and secondary Immunofluorescence antibodies in monoclonal and polyclonal formats as well as conjugated to fluorescent tags including fluorescein isothiocyanate (FITC), Green fluorescent protein (GFC), Alexa-fluor and more.
Some of our most popular Immunofluorescence antibodies include:
Adenovirus antibody (FITC): Adenoviruses are responsible for 5–10% of upper respiratory infections in children, and many infections in adults, this FITC conjugated monoclonal Adenovirus antibody is one of our most popular infectious disease Immunofluorescence antibodies.
E. coli O157 antibody: E coli 0157 is a common cause of food poisoning and diarrhoea in humans, this protein A purified E. coli O157 antibody is suitable for use in ELISA and Immunohistochemistry as well as Immunofluorescence.
Chlamydia antibody (FITC): Chlamydia infections are the most common bacterial sexually transmitted infections in humans and are on the rise worldwide. This FITC conjugated monoclonal Chlamydia antibody is an excellent choice for Immunofluorescence applications.
Thyroglobulin antibody (FITC): Thyroglobulin testing is primarily used as a tumor marker to evaluate the effectiveness of treatment for differentiated thyroid cancer and to monitor for recurrence. This conjugated thyroglobulin antibody is ideal for both Immunofluorescence and Immunohistochemistry procedures.
Donkey anti Rat IgG (Alexa Fluor 594): This immunoglobulin is one of our most popular Alexa-fluor secondary antibodies, it gives excellent results in ELISA and Electrophoresis as well as Immunofluorescence.
Check out our Immunofluorescence Protocol for some handy tips for doing immunofluorescence based assays.