Recognizes endogenous levels of AP2 alpha/beta protein
Cross Reactivity
Mouse, Rat, Chicken, Pig, Sheep
Applications
ICC, IF, IHC, WB
Immunogen
AP2 alpha/beta antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AP2 alpha/beta as the immunogen
Western blot analysis using AP2 alpha/beta antibody (70R-50465)
Western blot analysis of AP2 alpha/beta expression in HeLa (A); HepG2 (B); mouse brain (C); rat kidney (D) whole cell lysates.
AP2 alpha/beta antibody
Western blot analysis using AP2 alpha/beta antibody (70R-50465)
Western blot analysis of AP2 alpha/beta expression in HeLa (A); HepG2 (B); mouse brain (C); rat kidney (D) whole cell lysates.
ChIP analysis using AP2 alpha/beta antibody ( 70R-50465)
ChIP analysis of Cervical cancer cell lines lysate incubated for 12 hours at 4 C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Detection step: Semiquantitative PCR. Positive control: Tumor cell lines Hela. Negative control: Human primary keratinocytes.
Immunohistochemical analysis using AP2 alpha/beta antibody (70R-50465)
Immunohistochemical analysis of AP2 alpha/beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using AP2 alpha/beta antibody (70R-50465)
Immunofluorescent analysis of AP2 alpha/beta staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
AP2 alpha/beta antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
AP2 alpha/beta seems to play in important role in human breast cancer. AP2 alpha is the only AP2 protein required for early morphogenesis of the lens vesicle. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.