APAF1 antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence at the N-terminal of human APAF1, identical to the related Rabbit sequence, and different from the related rat sequence by one amino acid, as the immunogen
Optimal conditions to be determined by the end user
Storage & Safety
Storage
Store at 4 deg C for short term storage. For long term storage aliquot and store at -20 deg C, avoid repeated freeze/thaw cycles.
General Information
Biological Significance
During apoptosis, APAF 1 (apoptosis protease activating factor 1) binds to Cytochrome C (APAF 2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro caspase 9, and activates caspase 9 (APAF 3). Activated caspase 9 in turn cleaves and activates caspase 3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF 1 is ubiquitously expressed in human tissues.